A younger son of the treasurer of Elizabeth’s Household, Knollys was also a nephew of the queen through his maternal grandmother, a sister of Anne Boleyn. In 1586-7, he commanded the lifeguard of his brother-in-law Robert Dudley, earl of Leicester, from whom (as governor-general of the Low Countries) he received his knighthood.
Knollys’s father had been a religious reformer under Edward VI, and spent the latter part of Queen Mary’s reign with the English exile church at Frankfurt. In 1618 Thomas Taylor, lecturer at Reading, dedicated his Christs Combate to Knollys, commending him as ‘a worthy instrument in this place [Reading], which as well by your authority and care, as through your godly affection and countenance of good men and causes hath a long time enjoyed much comfort, assistance and refreshing’.
Knollys first entered Parliament on his father’s interest as high steward of Oxford, at a by-election caused by the death of his elder brother Edward in Ireland; he continued to represent the borough until his father’s resignation from the stewardship in 1592. In 1585 he was granted a lease of the Crown manor of Lewisham, adjacent to Greenwich Palace, which he held for most of his life,
While Knollys is not recorded as having spoken in the patchily recorded debates of 1604-10, he was named to numerous committees. Only a few held any political significance: his nomination to attend two conferences with the Lords concerning the Union (14 Apr. 1604 and 24 Nov. 1606) probably had as much to do with his eldest brother’s recent elevation to the peerage as any personal interest in the subject;
As might have been expected, Knollys showed some interest in religious matters. In 1604, one of the first committees to which he was named (19 Apr.) was charged to prepare for a conference with the Lords on the provision of adequate maintenance for a learned ministry, and to consider Sir Edward Montagu’s* complaints against ecclesiastical courts.
Knollys’s committee appointments demonstrate some links to local and personal concerns. His nomination to the committee for the bill to raise the statutory maximum for the length of kersies (5 Feb. 1606) may have been intended to further the interests of the Reading cloth trade.
After 1610 Knollys remained active in local affairs. Indeed, he was appointed a trustee for the borough of Hungerford in 1613.
As a deputy lieutenant, Knollys was expected to help implement the Forced Loan, but he failed to attend one of the first meetings of the Loan commissioners,
Knollys and his son Sir Francis II* were returned to the Short Parliament for Reading a few days before its dissolution, in opposition to (Sir) Robert Heath*, who was probably the nominee of the high steward, Knollys’s great-nephew, the earl of Holland (Henry Rich*). They defeated another of Holland’s candidates in the election for the Long Parliament,
